Abstract: The equation of state for asymmetric nuclear matter (NM) is determined essentially by the isospin dependence of the NN interaction which isoriginated from the neutron-proton asymmetry in the medium. To explore isospin effects, asymmetric NM has been studied within the Hartree Fock formalism using different density-dependent M3Y interactions as well as the Skyrme (SLy4) and Gogny (D1S, D1N) interactions. While all these interactions were proven to be quite realistic in the nuclear structure or reaction studies, they are clearly divided into two families which are associated with different behaviors of the NM symmetry energy at high densities. As a consequence, the two families offer two distinct scenarios for the proton-to-neutron ratio in beta-equilibrium which imply two drastically different mechanisms for the neutron star cooling. Such an ambiguity in the NM symmetry energy at high densities remains an open question due to the experimental evidences favoring either families of the mean-field interactions. In finite nuclei, the neutron-proton asymmetry is largest at the surface of neutron-rich nuclei which can significantly affect the shell evolution of valence neutrons. To study such effects, a coupled-channel (CC) analysis of the $^{18,20,22}$O$(p,p')$ data has been performed to determine the neutron transition strengths of 2$^+_1$ states in $^{18,20,22}$O, using the microscopic optical potential and inelastic form factor given by the folding model. With the isospin dependence of the effective interaction carefully fine-tuned against the data of charge-exchange (p,n) reactions, the isoscalar (delta_0) and isovector (delta_1) deformation lengths of 2$^+_1$ states in $^{18,20,22}$O were extracted from the CC analysis of (p,p') data. A specific N dependence of delta_0 and delta_1 has been established which can be linked to the neutron shell closure occurring at N-> 16. The ratios of the neutron/proton transition matrix elements (M_n/M_p) determined for the 2$^+_1$ states in $^{18,20}$O have been compared to those deduced from the mirror symmetry, using the measured B(E2) values of 2$^+_1$ states in the proton rich $^{18}$Ne and $^{20}$Mg nuclei, to discuss the isospin impurity in the $2^+_1$ excitation of the A=18,T=1 and A=20,T=2 isobars.